Jamestown was a settlement in the Colony of Virginia, the first permanent English settlement in the Americas. Established by the Virginia Company of London as "James Fort" on May 24, 1607 (O.S., May 14, 1607 N.S.), and considered permanent after brief abandonment in 1610, it followed several earlier failed attempts, including the Lost Colony of Roanoke. Jamestown served as the capital of the colony for 83 years, from 1616 until 1699.
Some of the hardships where: Isolation. Living in a new world with no support outside of what they brought. Indians and a language barrier. Unpredictable weather. they had no idea what the seasons would be like as they knew no one who had lived there. Disease. No way to effectively treat medical problems even with the rudimentary medical practices of the time.